P-01-06-002
Monosex strain development for white sturgeon (Acipenser tranmontanus) and salmonids

Abstract:

The project encompass applied and basic studies examining the mechanism of sex differentiation in sturgeon and several salmon species, with the goals of developing monosex strains.

For sturgeon, the project will examine the timing of sex determination and differentiation, the mechanism of sex determination using gynogenesis to determine the window of sensitivity for sex reversal by different sex steroids and methods for phenotypic sex identification of sturgeon using ultrasound. Assays for sturgeon to allow identification of egg-producing individuals will then be developed.

For coho and Atlantic salmon, a quantitative PCR diagnostic test will be developed for simplified genetic sex identification methodology for coho and chinook salmon. Continue efforts will be made to characterize the salmonid Y chromosome at the molecular level to search for sequences which are conserved among all salmonid species and can be used for universal testing of genetic sex to produce monosex Atlantic salmon strains available for use in the BC aquaculture industry.

For all species improved diagnostic tools for characterizing sex differentiation including nucleic acid and/or antibody tests for male gonadal cells will be developed.

Project Start Date: November 01 2001
Project End Date: June 30 2004

Contact: Robert Devlin (DevlinR@pac.dfo-mpo.gc.ca)