Identification of immunoreactive peptides to function as vaccine candidates in a recombinant subunit vaccine against Loma salmonae

P-06-01-003

Description

  • Obtain full length cDNA sequence data for 10-15 L. salmonae genes by creating a subtractive, normalized cDNA library and sequencing 96 clones.
  • Compare the genes uncovered using genomic and proteomic approaches, and attempt to isolate from cDNA "important" genes that were identified in peptide surveys.
  • Identify and synthesize peptide sequences which have adjacent or overlapping B- and T-cell epitopes.
  • Test peptides for immunoreactivity using convalescent antiserum and by their ability to stimulate proliferation of peripheral blood leukocytes from recovered chinook salmon.
  • Rank peptides for their suitability as candidates to be incorporated into a recombinant subunit vaccine.

Program Name

Aquaculture Collaborative Research and Development Program (ACRDP)

Year(s)

2006 - 2007

Ecoregion(s)

Pacific: Vancouver Island West Coast

Principal Investigator(s)

Valerie Funk
Email: ValFunk@telus.net